THE INFLUENCE OF ADAPTIVE HORMONES ON THE CONTENT OF MAGNESIUM IN THE PANCREATIC ISLETS, THYMUS AND BLOOD LYMPHOCYTES OF ANIMALS WITH STREPTOZOTOCIN-INDUCED DIABETES
DOI:
https://doi.org/10.32782/naturaljournal.4.2023.1Keywords:
insular apparatus, immune system, magnesium, functional connections.Abstract
At the heart of diabetes, which is one of the most common diseases of the endocrine system, are deep disorders of metabolism, including mineral metabolism. The relevance of studies of the content of intracellular magnesium is due to the importance of this metal for the functioning of the insular apparatus and the immune system, as well as the involvement of the latter in the mechanisms of the development of insulin-dependent diabetes mellitus. The aim of the study was to study changes in magnesium content in pancreatic islets, thymus, and blood lymphocytes of animals with streptozotocin-induced diabetes when adaptive hormones (insulin, adrenaline, and prednisolone) were administered. The development in our laboratory of the cytochemical reaction of lumomagnesone to magnesium in cells of the blood, pancreas and thymus allowed us to conduct such studies. Correlation analysis was performed to assess the degree of connection between changes in the studied indicators. It was established that administration of the diabetogenic agent streptozotocin led to the development of magnesium deficiency in pancreatic β-cells, thymic epithelial cells, and blood lymphocytes of mice and rats. Administration of adaptive hormones partially corrected magnesium deficiency in the studied cells of diabetic animals. In experimental mice and rats, a more pronounced effect was observed in the case of combined action of these hormones. The conducted correlation analysis proved a reliable positive correlation of changes in the amount of metal in the studied animal cells, which indicates the presence of close immune-insular relations. The practical significance of the work is that the obtained results can be used to refine the algorithm for the diagnosis of diabetes mellitus and further carry out pathogenetic treatment measures for this disease.
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